Journal of Hubei University for Nationalities - 2013

Objective To investigate the effect of crocin on the proliferation and migration of CNE2 cells,and explore the underlying molecular mechanism.Methods Microscope was used to observe morphological changes of CNE2 cells after cultured with different concentrations of crocin.CCK-8 experiment was used to examine the inhibition of crocin on the proliferation of CNE2 cells.Transwell was used to determine the migration ability of CNE2 cells.RT-PCR reaction was used to test mRNA expression of bi-1 gene.Results After using different concentrations crocin for 24 hours,CNE2 cells was suppressed both in proliferation and migration ability in a dose-dependent way.But when the crocin concentration is higher than 0.8 mmol/L,cell morphology would significantly change,or even died.RT-PCR reaction showed that bi-1 gene mRNA expression in CNE2 cells was down-regulated after being treated by different concentrations of crocin.Conclusion Crocin can inhibit the proliferation and migration of CNE2 cells,and can affect the expression of anti-apoptosis gene bi-1.Further experiments illustrated that the inhibition is probably induced by regulation of bi-1 gene.